Biology Reference
In-Depth Information
sections and were defined by the presence of a discontinuity in the postsynaptic density
(Geinisman et al., 1987). To minimize subjectivity, classification was carried out blind by at
least two experimenters and if distinction was unclear, the synapse was not included in the
quantification.
Statistical analysis
The unpaired Student's t and Kruskal-Walis tests were used to detect significant
differences between sham lesioned group with 6-OHDA lesioned striatum; and the paired
Student's t test to detect differences between ipsi and contralateral striatum (p<0.05).
R
ESULTS
Diameters of synaptic endings
Sham lesioned rats did not show any differences between both striata, nor alterations at
the different stages after the surgery (figures 10 and 11-1).
Figure 10. A-B: Time course of changes for synaptic ending diameter (major (A) and minor (B) axes)
in ipsi and contralateral striatum following 6-OHDA and sham lesions. * P < 0.001. C: Mean of the
total number of synaptic endings establishing synapses with a dendritic spine. * P < 0.001. D: Mean of
the total number of perforated synapses in ipsi and contralateral striatum following 6-OHDA and sham
lesions. * P < 0.001
