Biomedical Engineering Reference
In-Depth Information
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30. Brady, G., Barbara, M. and Iscove, N.N. (1990) Representative in vitro cDNA amplification
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17-25. The first demonstration of our global-RT-PCR approach to the study of transcripts from
single cells.
31. Brady, G., Billia, F., Knox, J. et al . (1995) Analysis of gene expression in a complex differentiation
hierarchy by global amplification of cDNA from single cells. Current Biology , 5 , 909-922.
Application of the global-RT-PCR method to the study of heterogeneous populations of single
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32. Brady, G. and Iscove, N.N. (1993) Construction of cDNA libraries from single cells. Methods in
Enzymology , 225 , 611-623. A detailed account of the utility of the global-RT-PCR method.
33. Dulac, C. and Axel, R. (1995) A novel family of genes encoding putative pheromone receptors in
mammals. Cell , 83 , 195-206. Generation of single cell source cDNA libraries and downstream
methods.
34. Iscove, N.N., Barbara, M., Gu, M. et al . (2002) Representation is faithfully preserved in global
cDNA amplified exponentially from sub-picogram quantities of mRNA. Nature Biotechnology ,
20 , 940-943. The first application of our global-RT-PCR methodology for microarray-based gene
expression analysis.
35. Tietjen, I., Rihel, J.M., Cao, Y. et al . (2003) Single-cell transcriptional analysis of neuronal
progenitors. Neuron , 38 , 161-175. Application of a global-RT-PCR strategy to gene expression
analysis using Affymetrix GeneChips™.
36. Jensen, K. and Watt, F. (2006) Single-cell expression profiling of human epidermal stem and
transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence. Proceedings of the National
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37. Kurimoto, K., Yabuta, Y., Ohinata, Y. et al . (2006) An improved single-cell cDNA amplification
method for efficient high-density oligonucleotide microarray analysis. Nucleic Acids Research ,
34 , e42. An extension of the global-RT-PCR technique to increase robustness.
38. Kurimoto, K., Yabuta, Y., Ohinata, Y. and Saitou, M. (2007) Global single-cell cDNA amplifi-
cation to provide a template for representative high-density oligonucleotide microarray analysis.
Nature s , 2 , 739-752. An extension of the global-RT-PCR technique to increase robustness.
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cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis.
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