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respectively) functional groups and carbonyl C=O ester (ca. 1745 cm (-1)
spectral intensities as well as their ratios of antisymmetric CH(3) to
antisymmetric CH(2) (Ratio of CH
3
as to CH
2
as), ratios of symmetric
CH(3) to symmetric CH(2) (Ratio of CH
3
s to CH
2
s), and ratios of
carbonyl C=O ester peak area to total CH peak area (Ratio of C=O ester
to CH). Together with the multivariate molecular spectral analyses, the
study focused on detecting spectral feature of molecular structure
functional groups that are related to lipid biopolymer in different types of
peas.
Keywords:
Nutrition-structure interactions, yellow and green types of peas,
cultivation, functional groups, lipid conformation, structural modification
and spectral features
1.
I
NTRODUCTION
Two types of green-type and yellow-type of peas (
Pisum sativum)
varieties have been developed by Crop Development Center (CDC, the
University of Saskatchewan, Saskatoon), such as Green-Type: cv. CDC Strike
and Yellow-Type: cv. CDC Meadow (Warkentin et al., 2007; The Western
Producer, 2014).
To date, there is limited study on structural profiles on a molecular level
mainly related to lipid biopolymers in different type of peas. This research
aims to determine molecular structural features of lipid that mainly related to
functional groups in the recently developed Green-type and Yellow-type CDC
pea varieties: CDC Strike (Green-Type) vs. CDC Meadow (Yellow-Type) and
relatively quantify the antisymmetric CH3 (“CH
3
as”) and CH2 (“CH
2
as”) (ca.
2960 and 2923 cm (-1), respectively), symmetric CH3 (“CH
3
s”) and CH2
(“CH
2
s”) (ca. 2873 and 2954 cm (-1), respectively) functional groups and
carbonyl C=O ester (ca. 1745 cm (-1) spectral intensities as well as their ratios
of antisymmetric CH(3) to antisymmetric CH(2) (Ratio of CH
3
as to CH
2
as),
ratios of symmetric CH(3) to symmetric CH(2) (Ratio of CH
3
s to CH
2
s), and
ratios of carbonyl C=O ester peak area to total CH peak area (Ratio of C=O
ester to CH).
The multivariate molecular spectral analyses, cluster analysis and
principal component analysis of original spectra (without spectral
parameterization), distinguished the structural differences in the antisymmetric
and symmetric CH(3) and CH(2) spectral region (ca. 3001-2799 cm(-1)) and
carbonyly C=O ester band region (ca. 1771-1714 cm(-1)). The emphasis of
this study focused on how molecular structure functional groups that are
