Agriculture Reference
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related to lipid biopolymer could be detected using molecular spectroscopic
techniques with multivariate techniques.
2.
L
IPID
R
ELATED
M
OLECULAR
S
TRUCTURAL
S
PECTRAL
F
EATURES OF
G
REEN
T
YPE AND
Y
ELLOW
-T
YPE OF
CDC
P
EAS
(
P
ISUM SATIVUM
):
C
OMPARISON
B
ETWEEN
D
IFFERENT
T
PES
The molecular structures can be detected by advanced molecular
spectroscopy such as FT/IR-ATR molecular spectroscopy (Yu, 2010; Samadi
and Yu, 2011). In this project, we aimed to use univeriate molecular spectral
analysis method to determine molecular structural changes of lipid related
functional groups in the Green-type and Yellow-type CDC pea varieties.
2.1. Green-Type and Yellow-Type of CDC Peas (
Pisum sativum
)
The Green-type and Yellow-type CDC pea varieties: CDC Strike (Green-
Type) vs. CDC Meadow (Yellow-Type), were provided by Professor Dr. Tom
Warkentin, Crop Development Centre (CDC, University of Saskatchewan,
Saskatoon, Canada).
2.2. Green-Type and Yellow-Type of CDC Peas (
Pisum sativum
):
Univariate Approach
Molecular functional groups that are related to lipid biopolymer in the
green-type and yellow-type of CDC peas (
Pisum sativum
) were identified
according to published reports (Himmelsbach et al., 1998; Wetzel et al., 1998;
Marinkovic and Chance, 2006; Yu, 2011) and analyzed using univariate
molecular spectral analysis (Yu, 2004).
Figure 1 shows typical spectrum in
green-type (CDC Strike) of peas.
Functional groups that are related to lipid biopolymers included
antisymmetric CH
3
(at ca. 2960 cm
-1
), antisymmetric CH
2
(at ca. 2923 cm
-1
),
symmetric CH
3
(at ca. 2873 cm
-1
) and symmetric CH
2
(at ca. 2853 cm
-1
), and
lipid carbonyl C=O ester group (at ca. 1745 cm
-1
). The carbonyl C=O ester
group and antisymmetric and symmetric CH
2
and CH
3
stretching functional
