Agriculture Reference
In-Depth Information
B
ETA
-1,3-G
LUCANASE
A cDNA encoding a beta-1,3-glucanase, named PsGNS2, has been
isolated. PsGNS2 expression is detected in young pea flowers and seed coat
but there is low expression in roots and stems during development of the pea
seedling. PsGNS2 induction is not seen following environmental stress or
fungal infection (Buchner et al., 2002).
S
YMBIOSIS
-R
ELATED
C
HITINASES
A quadruple of acidic symbiosis-related 30-kDa chitinase isoforms with
isoelectric points ranging between 5.2 and 5.85 has been induced after
colonization of
Pisum sativum
L. cv. Frisson roots with Glomus mosseaef, an
arbuscular mycorrhizal fungus. One of these isoforms sclosely resembles a pea
shoot class-I chitinase isoform (Slezack et al., 2001).
B
OWMAN
-B
IRK
P
ROTEASE
I
NHIBITORS
A Bowman-Birk family of plant trypsin inhibitor (BBI) isoforms purified
from pea seeds demonstrate an IC50 of 41 microM to the pea aphid
Acyrthosiphon pisum
and a LC50 of 48 microM at 7 days. Cyclic peptides
with the Bowman-Birk anti-chymotrypsin head are much more toxic and
growth inhibitory than the anti-trypsin counterparts. The toxicity is witnessed
in restlessness of aphids fed with the toxic peptides. However, chymotrypsin
activity is indiscernible in the aphid gut, and the target of the chymotrypsin
inhibitor awaits elucidation (Rahbé et al., 2003).
A
NGIOTENSIN
C
ONVERTING
E
NZYME
(ACE)
I
NHIBITORY
P
EPTIDE
Following fermentation of pea seeds by
Lactobacillus plantarum
299v in
monoculture and digestion under gastrointestinal conditions, the sequence of
the ACE inhibitory peptide obtained from pea proteins has been determined
and found to be DDEEEEQGEEE (Jakubczyk et al., 2013).
