Agriculture Reference
In-Depth Information
A
NTIFUNGAL
P
ROTEINS
Pisum sativum
var.
macrocarpon
(sugar snap, also called honey pea)
legumes produce sativin, a 38-kDa antifungal protein with an N-terminal
sequence analogous to those of miraculin (a sweet plant protein) and pisavin (a
ribosome-inactivating protein from
Pisum sativum
var. arvense Poir displaying
similarity to miraculin). Sativin manifests antifungal activity against
Coprinus
comatus, Fusarium oxysporum,
and
Pleurotus ostreatus
but not against
Rhizoctonia solani
(Ye et al., 2000). A31-kDa antifungal protein designated
pisumin, also from the sugar snap pea, exhibits antifungal activity against
C.comatus
and
P.ostreatus
and attenuated activity against
F. oxysporum
and
R.
solan
i. (Ye and Ng, 2003).
P
EA
A
LBUMIN
1F
(PA1F)
The peptide pea albumin 1F (PA1F) produces a hyperglycemic action
following subcutaneous injection to normal and type II diabetic mice (KK/upj-
Ay) at a dosage of 5 or 10 microg/g body weight. PA1F binding protein from
murine pancreatic cell membrane, same as the voltage-dependent anion
channel 1 (VDAC-1), may play a role in blood glucose regulation after PA1F
binding (Dun et al., 2008).
P
EA
A
LBUMIN
1,
S
UBUNIT B
PA1b (pea albumin 1, subunit b) is a cysteine-rich pea seed defense
protein with 37 amino acids. It induces short-term mortality in the cereal
weevils
Sitophilus granarius
,
S. oryzae
, and
S.zeamais
). The primary sequence
of the protein does not share similarities with other known toxins. PA1b
belongs to the inhibitor cystine-knot family with six cysteines forming three
disulfide bridges. It displays a triple-stranded antiparallel beta-sheet with a
long flexible loop linking the first to the second strand and a series of turns.
The lipophilic and electrostatic potentials at the surface of PA1b are very close
to those found in the spider toxin ACTX-Hi:OB4219, thus indicating activity
on ion channels (Jouvensal et al., 2003).
