Biomedical Engineering Reference
In-Depth Information
1.2
(A)
(B)
1.8
1.6
1.0
1.4
0.8
1.2
1.0
24.3
µ
m
0.6
17.92
µ
m
0.8
0.4
0.6
0.4
0.2
0.2
0.0
0.0
1.6
(C)
(D)
1.4
1.4
1.2
1.2
1.0
1.0
30.2
µ
m
26.02
µ
m
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
Figure 7.6
Atomic-force microscope images of live cells after 24 h of cell culture: (A) on non-
patterned arginine-glycine-aspartate (RGD) monolayer; (B) on two-dimensional RGD nanodot; (C) on
three-dimensional RGD nanorod; (D) on three-dimensional RGD nanopillar arrays. Scale bar 5
µ
m.
Figure reproduced with permission from: ref. 48, © 2012 Elsevier.
time at a constant voltage during the mask fabrication process, while the structure of the peptide
was controlled by concentrations of RGD peptide solution, self-assembly times, and pore sizes.
Cell functions on the three-dimensional RGD-MAP (multiple-arm peptide)-Cys (cysteine)
nanopatterned surfaces were significantly increased compared with those on a RGD-MAP-Cys
monolayer and two-dimensional nanodot surface, regardless of the cell line. Among the
three-dimensional peptide nanostructures, the three-dimensional nanopillar array was found to
be more suitable for cell adhesion and spreading than the three-dimensional nanorod array
(Figure 7.6) due to the increased binding sites for the integrin receptor on the cell surface, that
contributes to the formation of a strong link between the cells and Au.
Application of Nanopatterned Surface to Stem-Cell-Based Chips
Cell-based chips hold great promise as an effective
in vitro
tool for assessing the effects of
environmental toxins, anticancer drugs or inorganic particles on the target living cells with high
sensitivity [49-51], and for confirmation of cell-cell interactions to overcome the disadvantages
