Chemistry Reference
In-Depth Information
Table 7.1
(Continued)
Receptor
Ligand
Technique
Receptor mobility and ligand binding properties
Reference
TCR
and
CD8
tagged with
Peptide MHC tetramer
FCS and time-
resolved
Diffusion: Heterogeneous diffusion of both CD8 and TCR
in the plasma membrane. Two populations of TCR and
CD8 receptors with different mobility were observed. Some
of the CD8 and TCR molecules are colocalized in mem-
brane domains.
[172]
uorescently labeled antibody
in T-cells
uorescence
Binding: The time-course of peptide MHC binding to live T
cells was biphasic. A fast association to CD8 preceded
association of the peptide MHC-CD8 complex with TCR.
TrkA receptor
labeled with QD
coated with anti-TrkA antibody
in neural PC12 cells
RTA anti-TrkA that acts as a
partial agonist
NGF
SMI
Diffusion: Tracking of activated TrkA receptors revealed
endocytosis and active transport via microtubules to dif-
ferent subcellular locations.
[173]
a
The nature of the benzodiazepine receptor was not described in this reference.
b
No evidence for direct interaction between GABA
A
R and kavain was reported in this study, only the speci
c binding of kavain to cortical neurons was demonstrated.
Abbreviations for receptors: 5-HT
3
R, serotonin type 3 receptor; A1-AR, A1-adenosine receptor; AMPAR,
a
-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
receptor;
b
2
-AR,
b
2
-adrenergic receptor; cAMPR, cyclic adenosine 3
0
,5
0
-monophosphate receptor; EGFR, epidermal growth factor receptor; ET
A
R, endothelial A receptor;
GABA
A
R,
-aminobutyric acid class A receptor; IgE receptor, immunoglobulin E receptor; LFA-1, leukocyte function-associated antigen-1; mGluR 5, metabotropic
glutamate receptor 5; MHC receptor, major histocompatibility complex receptor; NGFR, nerve growth factor receptor; NK1R, neurokinin 1 receptor; OR17-40, human
odorant receptor 17-40; SSTR1, somatostarin type 1 receptor; SSTR5, somatostarin type 5 receptor; TCR, T-cell receptor.
Other abbreviations: CHO, Chinese hamster ovary; FCS,
uorescence correlation spectroscopy; FIDA,
uorescence intensity distribution analysis; FRET,
uorescence
resonance energy transfer; GPI, glycosyl phosphatidylinositol; HEK, human embryonic kidney; ICAM-1, intercellular adhesion molecule-1; PMA, phorbol-12-myristate-
13-acetate; Ncad-Fc, N-cadherin ectodomain fused to the IgG Fc fragment; QD, quantum dots; SMI, single molecule imaging; SPT, single particle tracking; TIRFM, total
internal re
g
ection
uorescence microscopy.
