Chemistry Reference
In-Depth Information
Binding: Interaction between Lyn and activated Fc
e
RI was
revealed by FCS cross-correlation.
Insulin receptor
in human re-
Insulin
labeled with
tetramethylrhodamine
FCS
Diffusion: Two populations of ligand
-
receptor complexes
[169]
nal tubular cells
with different mobility.
Binding: Insulin binding isotherm yielded two binding
processes: a low af
nity with K
d
¼1 nM and stoichiometry
1:1 (n
10
2
nM and
¼
1), and a high af
nity with K
d
¼
5
·
n
<
1.
N-cadherin receptor
in myo-
Ncad-Fc
ligand conjugated to
SPT
Diffusion: The kinetics of the anchoring of N-cadherin to the
actin cytoskeleton on lamellipodia was studied. The
movement of ligand-coated beads showed an initial freely
diffusing phase. The anchoring latency is lower at high
ligand density at the bead surface.
[170]
genic cells
1-
m
m latex beads
NGFR
(TrkA or/and p75 re-
ceptors) in dorsal root ganglion
growth cones
NGF
labeled with Cy3
SMI
Diffusion: Two diffusion modes for NGFR were observed:
Brownian motion and one-directional movement toward
the central region of the growth cone. Ligand
[171]
receptor
complexes were transported toward the central region by
the rearward
-
ow of the actin network. The endocytosis of
individual complexes (no need for cluster formation) was
evidenced by exposure to an acidic medium.
Binding: Counting the number of
uorescent dots revealed
that lamellipoidal expansionwas initiated by 40 bound Cy3-
NGF. On high-af
10
2
nM and
nity receptors, K
d
¼
2.7
·
B
max
¼
175 bound ligands on a single growth cone.
(Continued)
