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Fig. 6
Suggested timetable of steps in the procedure.
transcripts per cell. This approach should be useful for laboratories that wish to make
a semiquantitative determination of gene expression at a modest cost in C. elegans or
other related species.
Acknowledgments
The authors thank others who have helped them test and develop the protocol and provide advice about
reagents, in particular George Hsu, Andre Pires da Silva, Lenore Price, David Fitch, and Andrew Fire. The
protocol was developed through funding from the NSF (grants IBN#0416922, IOS#0643325) to Morris F.
Maduro.
References
Bobrow, M. N., and Moen, P. T., Jr. (2001). Tyramide signal amplification (TSA) systems for the
enhancement of ISH signals in cytogenetics. Curr Protoc Cytom Chapter 8, Unit 8.9. Available at
Broitman-Maduro, G., Owraghi, M., Hung, W. W., Kuntz, S., Sternberg, P. W., and Maduro, M. F. (2009).
The NK-2 class homeodomain factor CEH-51 and the T-box factor TBX-35 have overlapping function
in C. elegans mesoderm development. Development
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, 2735-2746.
Coroian, C., Broitman-Maduro, G., and Maduro, M. F. (2005). Med-type GATA factors and the evolution
of mesendoderm specification in nematodes. Dev. Biol.
289
, 444-455.
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