Enzymatic Synthesis of Heparin - Sustainable Biotechnology: Sources of Renewable Energy - page 268

NDST2, Heparitinase I

Hexasaccharide 1

NS NS

Epimerase/2-OST

NS 2S NS

6OST-1/6OST-2a

6S NS6S 2S NS

Δ 4, 5 -Glucuronidase

NS6S

6S 2S NS

3-OST-1, PAP 34/35 S

GlcNAc

GlcUA IdoUA

Δ UA

NS6S3 34/35 S 6S 2S NS

Fig. 5 Enzymatic synthesis of AT binding pentasaccharide. The synthesis was begun with heparosan. Six enzymatic steps were utilized to synthesize an AT
binding pentasaccharide with anticoagulant activity. Either PAP 34 SorPAP 35 S was used in 3OST1 modification step for structural characterization by ESI-MS