Biomedical Engineering Reference
In-Depth Information
injected to the ACE, 5μm 250×4.6 mm analytical column with the mobile
phase as the same as elution solvent. h is method was used for extraction
of carbamazepine from an anticonvulsant aqueous solution and the results
revealed an extraction recovery of more than 90%. HPLC chromatograms
show an ei cient clean up, which supports the potential of MI-solid phase
extraction technique for clean-up of trace amounts of carbamazepine from
the drug formulation.
Ivanova-Mitseva
et al.
reported for the i rst time the synthesis of cubic
MIP with l uorescent core structure [86]. For the i rst time cubic organic
NPs are reported. Cubic NPs were an unexpected result and the origin of
this morphology could be the subject of future study, however this shape
may be an advantage where close packing of NP-based material is required,
such as in dense coatings. Fluorescent-core cubic MIPNPs were prepared
in an innovative concept. h ey demonstrated excellent selectivity and ai n-
ity at er just 10min incubation time. Advantages such as precise control of
the number of the l uorescent labels per particle and its polymer shielding
are reported for the i rst time as a new technique. NPs prepared in this way
are promising materials to replace antibodies in sensors and immunoas-
says and in drug delivery and diagnostics.
11.3.2 Nanosphere
Esfandyari-Manesh
et al.
reported a synthetic condition of precipitation
polymerization to obtain uniformly sized molecularly imprinted nano-
spheres of dipyridamole for application in the design of new drug deliv-
ery systems [87]. In addition, the morphology, drug release, and binding
properties of MIPs were studied, and the ef ects of morphology on other
properties were investigated. h e MIPs prepared by acetonitrile/chloro-
form (19:1, v/v) were uniformly sized nanospheres with an average mean
diameter of approximately 88 nm at a wetted state, 50 nm at a dry state,
and a polydispersity index of 0.062. h e imprinted nanospheres showed
excellent binding properties and had 62.7% of template binding compared
with 17.1% of its blank polymer. h e imprinted nanospheres with 67.5 (mg
template/of polymer) of binding capacity had better imprinting ei ciency
than the 50.5% of binding capacity shown by irregularly shaped MIP par-
ticles that were prepared by chloroform. h e molecular binding abilities of
imprinted nanospheres in human serum were evaluated by HPLC analysis
(binding about 77% of dipyridamole). Results from release experiments
of MIPs showed a very slow, controlled, and satisfactory release of dipyr-
idamole. h e loaded drug was released up to 99% in 17 days for nano-
spheres and 22 days for irregularly shaped particles.
