Biomedical Engineering Reference
In-Depth Information
compared to the carbon paste electrode, modii ed with non-imprinted
polymer (NIP (nano)-CP). It response ranges of 4×10
-12
-1×10
-10
M and
1×10
-9
-1×10
-7
M with the sensitivities of 31.7 and 0.17 μA nM
-1
, respec-
tively. h e lower detection limit of the sensor was calculated equal to
2.8×10
-12
M (S/N). h e sensor was applied for promethazine (PMZ) deter-
mination in plasma samples without applying any sample pre-treatment.
Similarly, the author has also reported a potentiometric sensor based on
the nano-sized molecularly imprinted polymer (MIP) for the determina-
tion of promethazine [83]. h is MIP nanoparticles were prepared by two
method micro emulsion polymerisation and suspension polymerisation
regarded as nano-MIP (1) and nano-MIP (2). h e nano-MIP (2) based
sensor showed higher selectivity and sensitivity, compared to the nano-
MIP (1) based electrode. Both electrodes demonstrated a response time
of 5s, a high performance and a satisfactory long-term stability. h e elec-
trodes were applied for PMZ determination in syrup and serum samples.
Asadi
et al.
used novel synthetic conditions of precipitation polymer-
ization to obtain nanosized cyproterone molecularly imprinted polymers
for application in the design of new drug delivery systems [84]. h e scan-
ning electron microscopy images and Brunauer-Emmett- Teller analy-
sis showed that MIP prepared by acetonitrile exhibited particles at the
nanoscale with a high degree of monodispersity, specii c surface area of
246 m
2
g
−1
, and pore volume of 1.24 cm
3
g
−1
. Controlled release of cyproter-
one from nanoparticles was investigated through in vitro dissolution tests
and by measuring the absorbance by HPLC-UV. h e pH dissolution media
employed in controlled release studies were 1.0 at 37
C for 5 h and then
at pH 6.8 using the pH change method. Results show that MIPs have a
better ability to control the cyproterone release in a physiological medium
compared to the NIPs.
Imprinted nanoparticles were also employed as packening material in
solid phase extraction technique. A new, simple, rapid, and sensitive solid-
phase extraction with MIP for determination of Carbamazepine in bio-
logical samples was developed by Akbari-adergani
et al
[85]. h ese nano
particle polymers were synthesized via a non-covalent molecular imprint-
ing approach through precipitation polymerization method using MAA
as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a
cross-linker agent, carbamazepine as a target template molecule and AIBN
as an initiator. h e optimal conditions for solid phase extraction technique
consisted of conditioning the cartridge using a pH=3.0 water, loading 5.0
ml of the sample under basic aqueous conditions, clean-up using 2×2ml
acetonitrile and elution with 3.0 ml methanol. At er optimization of solid
phase extraction technique procedure, an aliquot of extracted template was
°
