Biomedical Engineering Reference
In-Depth Information
pre-adsorption of
positively charged
template protein
self-assembly and subsequent surface
graft copolymerization in highly dilute
monomer solution of MAA, AAm,
DMAEMA, and MBA
template
removal
rebinding
Figure 11.13
Schematic illustration of the procedure for the imprinting of Lysozyme over
vinyl and carboxyl group modii ed silica nanoparticles via surface grat copolymerization
in highly dilute solution of functional and cross-linking monomers [Co-opted from
reference 32 with permission].
samples can be achieved by the molecular imprinting modii cation at the
surface of silica nanoparticles.
Surface imprinting over nanosized support materials is particularly
suitable for protein templates, considering the problems with mass trans-
fer limitation and low binding capacity [32]. Recently, Chen
et al.
studied
the synthesis process toward enhancement of the imprinting performance
by examining the ef ect of several synthesis conditions. Interestingly, the
feed crosslinking degree was found to have a great impact on the thick-
ness of the formed imprinting polymer layers and the recognition prop-
erties of the resulting imprinted materials (Figure 11.13). h e imprinted
particles with a crosslinking degree up to 50% showed the best imprint-
ing ef ect. h e imprinting factor achieved 2.89 and the specii c binding
reached 23.3 mg/g, which are greatly increased compared to those of the
lowly cross-linked imprinted materials reported previously. Moreover, the
relatively high crosslinking degree led to no signii cant retarding of the
binding kinetics to the imprinted particles, and the saturated adsorption
was reached within 10min.
11.2.1.4 Core-shell Nanoparticle
Core-shell nanocrystals and/or nanoparticles (CSNPs) are a class of mate-
rials, which have properties intermediate between those of small, individ-
ual molecules and those of bulk, crystalline semiconductors (Figure 11.14).
h ey are unique because of their easily modular properties, which are a
result of their size. h ese nanocrystals are composed of a quantum dot core
and a shell of a distinct semiconducting material. h e core and the shell are
