Biomedical Engineering Reference
In-Depth Information
TEOS
MPS
NH
3
⋅
H
2
O
MBAAm, APS,
TEMED
Fe
3
O
4
Fe
3
O
4
@SiO
2
Modified-Fe
3
O
4
@SiO
2
Fe
3
O
4
@SiO
2
@MIPs
Lysozyme
AAm
MAA
Rebinding protein
Removal protein
Sample
Luminuo,
CTMAB,
K
3
[Fe(CN)
6
]
NaCl
solution
Phosphate
buffer
Phosphate
buffer
96-well plate
Chemiluminescence analyzer
Washing
Loading
Conditioning
Magnet
Eluting
Figure 11.10
A schematic representation of Fe
3
O
4
@SiO
2
@MIPs preparation and CL
detection of lysozyme [Co-opted from reference 29 with permission].
Molecular imprinting is an attractive technique for preparing mimics of
natural, biological receptors. Nevertheless, the imprinting of macromol-
ecule remains a challenge due to their bulkiness and sensitivity to denatur-
ation. Jing
et al.
, have presented a method for preparing multifunctional
lysozyme-imprinted nanoparticles (magnetic susceptibility, molecular rec-
ognition and environmental response) [29]. h e magnetic susceptibility
was imparted through the successful encapsulation of Fe
3
O
4
nanoparticles.
Selective lysozyme recognition depended on molecularly imprinted i lm.
Moreover, it was also a hydrophilic stimuli-responsive polymer, which
could undergo a reversible change of imprinted cavity in response to a small
change in the environmental conditions. h us, m-MIPs had high adsorp-
tion capacity (0.11mg mL
−1
), controlled selectivity and direct magnetic
separation (22.1emu g
−1
) in crude samples. At er pre-concentration and
purii cation with m-MIPs nanoparticles, a sensitive chemiluminescence
method was developed for determination of lysozyme in human serum
samples (Figure 11.10). h e results indicated that the spiked recoveries
were changed from 92.5 to 113.7%, and the RSD was lower than 11.8%.
2.1.3 Silica Nanoparticles
Yu a n
et al.
reported the MIP layer of estriol at the silica nanoparticles
surface with selective recognition [30], where the methacryl groups of