Biomedical Engineering Reference
In-Depth Information
have been considered very ei ciently as interesting for immobilization of
desired biomolecules [79-81]. Immobilization of bioactive molecules onto
surface charged super paramagnetic nanoparticles (size
25 nm) is of
special interest noticed for bio-sensor, since magnetic behaviour of these
bioconjugates results in improved delivery and recovery of biomolecules
for desired bio-sensing applications and utility [72, 73, 82]. Besides all
these existing problem of aggregation and rapid biodegradation of Fe
3
O
4
nanoparticles onto a given amount of matrix containing biomolecules
will dei nitely be helpful in overcoming for modifying these nanoparticles
using CH by preparing hybrid nano biocomposite [82-89].
Fe
3
O
4,
the metal oxide nanoparticles have been considered very inter-
esting for immobilization of desired biomolecules (GOx) because of low
toxicity, strong superparamagnetic property, biocompatibility, etc. [90-92].
Immobilization of bioactive molecules on the surface of magnetic nanopar-
ticles is very signii cant, because magnetic behavior of these bioconjugates
is likely to improve delivery and recovery of biomolecules for biomedi-
cal applications [90]. With the recent advances in clinical diagnostics that
have been stimulately demanded for high sensitive and precise analytical
methods for estimation of desired analytes including glucose were as such
signii cantly important. It may be noted that the existing and upcoming
problem of estimation, aggregation, decomposition and rapid biodegrada-
tion of Fe
3
O
4
nanoparticles onto a desired matrix containing GOx could
be overcome by modifying these interesting magnetic nanoparticles using
materials such as conducting polymers, inorganic semiconductors and
biopolymers (polysaccharides), etc.
Sole
et al.
and Li
et al.
have reported Fe
3
O
4
-based magnetic immuno-
sensor for l owing injection and piezoelectric immune-sensor, respectively
for detection of immunoglobulin (IgG) [93, 94]. Cao
et al.
On the basis
of heme-proteins immobilized onto Fe
3
O
4
nanoparticles have fabricated
electrochemical bio-sensor [92]. Cao
et al.
have studied direct electron
transfer between the molecule of haemoglobin and pyrolytic graphite elec-
trodes enhanced and appreciated by Fe
3
O
4
nanoparticles in the layer to
layer self-assembly i lms where applicable [95]. Rossi
et al.
have studied
glucose oxidase - Fe
3
O
4
nanoparticles bio-conjugate for sensing of glucose
[72]. Kouassi
et al.
have investigated and inferred that attachment of ChOx
with carbodiimide activated Fe
3
O
4
nanoparticles and its response to cho-
lesterol [96]. Li
et al.
have fabricated and invented a renewable potentio-
metric immune-sensor which is based on immobilized anti-IgG into Fe
3
O
4
nanoparticles [97].Wei
et al.
have utilized Fe
3
O
4
nanoparticles both for glu-
cose detection and H
2
O
2
[98]. Chumming
et al.
had electrochemically syn-
thesized the Fe
3
O
4
a
prusian blue nanoparticles with core-shell structure
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