Biomedical Engineering Reference
In-Depth Information
Figure 3. Antonov et al. “Structural investigation of the interaction between bovine serum albumin and
acid gelatin in water”.
3.2.
E
FFECT OF
G
ELATIN ON THE
S
ECONDARY
S
TRUCTURE OF
BSA
(CD
S
PECTRA
)
CD can provide information about the secondary structure of proteins and nucleic acids
and about the binding of ligands to these types of macromolecules [28,29]. Figure 4 shows
far-UV CD spectra at 40
o
C for BSA and gelatin in water. Gelatin at +5
o
C had a positive
maximum peak at 221nm and a negative minimum peak at 192 nm, suggesting a typical triple
helical conformation, [30] whereas the positive peaks in gelatin at 40
o
C disappeared, showing
the random coils configuration.
Figure 4. Antonov et al. “Structural investigation of the interaction between bovine serum albumin and
acid gelatin in water”.
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