Biomedical Engineering Reference
In-Depth Information
Table 2. Wheat embryos of different age culture growth and development characteristic
(% of the total sample quantity)
Callus proliferation medium
Morphogenic calluses
with shoots
Regenerant yield
Genotype
with roots
NaCl,
0,42%
NaCl,
0,42%
NaCl,
0,42%
Control
Control
Control
Novosibirskaja 15
24,2
23,6
20,0
23,6
24,2
25,7
Taezhnaya
-
33,3
5,1
18,4
7,7
18,4
Novosibirskaja 15
26,1
21,1
11,6*
11,7*
20,3
11,7*
Taezhnaya
13,3
6,6*
-
-
-
-
*- significant difference between two embryo`s ages within the genotype (P≤0.05).
Table 3. Influence of NaCl on growth and development of wheat callus culture
Prolife
r
ating calluses, %
Regen
e
rating calluses, %
Rege
n
erant yield, %
Parental
genotype
NaCl, %
NaCl, %
NaCl, %
Co
Co
Co
0,42
0,84
1,68
0,42
0,84
1,68
0,42
0,84
1,68
64,1*
а
56,4*
а
25,7*
а
10,3*
а
18,0*
а
0,0*
а
Taezhnaya
83,8
92,1
43,3
50,0
32,4 42,1
Novosibirs-
kaja 15
46,2*
а
41,7
а
26,9*
а
12,5*
а
0,0*
а
76,0
69,2
56,0*
44,0
57,7
36,0 38,5
8,3*
а
38,5 53,9* 15,4*
а
8,3*
а
KS-1607
84,6
69,2
53,9*
45,0
46,2
61,6* 69,2*
50,0*
а
31,0*
а
20,9*
а
59,1 43,9* 23,8*
а
0,0*
а
Minusa
77,3
80,5
65,1
65,9
63,4
Average
data
77,8* 56,0*
а
41,9
а
16,6*
а
17,4*
а
2,08*
а
80,4
60,7*
49,8
58,2
41,5 44,6
Co - control medium without NaCl;
*- significant difference between control and selective media (P≤0,05);
а - significant difference between selective medium and medium with minimum selective level
(Р≤0,05).
Table 4. Influence of induced drought on growth and development
of wheat callus culture
Proliferating calluses, %
Regenerating calluses, %
Regenera
n
t yield, %
Parental
genotype
PEG
16%
PEG
22%
PEG
16%
PEG
22%
PEG 16%
PEG
22%
Control
Control
Control
16,7*
а
Taezhnaya
83,8
56,4*
50,0*
43,3
43,6
30,6
32,4
33,3
Novosibirs-
kaja 15
39,1
а
16,7*
а
76,0
37,5*
25,0*
44,0
75,0*
36,0
58,3*
75,0
а
25,0*
а
8,3*
а
KS-1607
84,6
50,0*
46,2
50,0
38,5
33,3
16,7*
а
Minusa
77,3
60,9
66,7
65,9
47,8*
42,9*
59,1
34,8*
Average data 80,4
51,2*
54,2*
49,8
54,1
34,4*
а
41,5
39,9
14,6*
а
*- significant difference between control and selective media (P≤0,05);
а - significant difference between selective medium and medium with minimum selective level
(Р≤0,05).
Search WWH ::
Custom Search