Biomedical Engineering Reference
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D level. They had equal proliferation activity at the second cultivation step. The most active
regenerant formation was detected at early cutivars culture, that are the most called-for under
Siberian field conditions.
Figure 1. Quantity of regeneration callus passaged from proliferation medium vary in 2.4-D level to the
different regeneration medium.
Table 1. Wheat callus culture growth characteristic (% of the total sample quantity)
Callus
induction
mediu
m
сallus proliferation medium
Regenerant yield
Morphogenic calluse
s
with shoots
proliferating
calluses
with roots
Genotype
NaCl,
0,42%
NaCl,
0,42%
NaCl,
0,42%
NaCl,
0,42%
Control
Control
Control
Control
Novosi-
birskaja 15
72,5
74,0
53,4
35,1
17,2
47,8
21,4
35,1
35,0
Taezhnaya
76,5
67,8
61,5
44,8
40,0
40,4
43,7
38,9
36,3
0,5
0,25
Minusa
40,8
77,5
43,2
-
15,9
38,4
15,9
-
15,9
KS-1607
56,7
59,8
73,7
-
26,7
-
26,7
-
20,0
Novosi-
birskaja 15
72,9
92,5*
78,0*
24,2*
23,6
20,0*
23,6
24,2*
25,7*
Taezhnaya
80,6
72,7
70,9*
-
33,3
5,1*
18,4*
7,7*
18,4*
1
0,5
Minusa
44,9
86,9
68,3*
4,8
-
15,9*
-
-
-
KS-1607
59,5
76,9*
54,2*
27,2
8,3*
27,2
8,3*
27,2
8,3*
13,8
13,7
13,8
15,6
14,1
LSD
0,05
for
factor
10,8
9,69
9,75
11,0
9,96
*- significant difference induced by 2.4-D concentration change within the genotype (P≤0.05).
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