Biomedical Engineering Reference
In-Depth Information
somaclonal variation, haploids, genetic transformation, virus elimination and practical skills
in tissue culture in the implementation of scientific research from the first course which
completes the head a research paper. Experiments use exclusive highly ornamental plants. In
particular studies on micropropagation of prospective plant Eustoma grandiflorum (Raf.)
Shinn has been carried out [11,13,14]. In this study, sterile culture were deposited in a bank
source material in vitro in the laboratory of Biotechnology used for our traditional breeding
and for research on those directions. We took explants from leaves, stems, nodes and roots in
experiments on the optimization of the mass proliferation of shoots. This technique is useful
for preservation important cultivars in vitro and micropropagation for different purposes. In
vitro storage is more convenient than storing in the greenhouse conditions and better than the
storage of seeds, because the lines are not completely inbred (homozygous), and the result
will not be identical to the parents.
From the analysis of materials literature shows that in Russia, similar studies had not
taken place on
Eustoma
. Promising areas of research on the culture were identified in the
students works [14]. There were following areas as more promising as model for use in this
work with other species:
Obtaining virus-free plant
Eustoma
grandiflorum
(Raf.) Shinn in meristem culture in
vitro.
Adventive regeneration
Eustoma
grandiflorum
leaves in sterile culture.
Getting the somaclonal variants of
Eustoma
from callus.
Reproduction
Eustoma
by
microcuttings.
Comparative analysis of propagation methods by
Eustoma
cuttings on the
physiological and economic characteristics of the plant.
Study of the conditions of propagation by cuttings of intact plants of
Eustoma
.
Development project on the use
Eustoma
in the landscape.
Creation of the collection of
Eustoma in vitro
for year-round production of rooted
cuttings.
The search for new mechanisms of recovery
Eustoma
virus (sterile culture of the
stem tops of
Eustoma
together with the tissues of plants producing anti-metabolites
Dianthus cariophyllus
L.,
Hypericum perforatum
L. et al).
Virological assessment of and other plants on Black Sea Coast of Sochi.
Development of acclimatization techniques ex vitro.
These directions are developed by students of the Department of Landscape
Construction in the University.
In the future, we will broad the range of rare plants for collections in vitro. The most of
them are tropical and subtropical ornamental plants which students study in the University on
the disciplines like "Southern Ornamental Plants - Dendrology and Floriculture”. For the
preparation of lectures on this discipline we uses materials of the world's latest researches.
Students learn the subject using original papers published in international scientific literature.
This discipline is linked very closely with the discipline of "Biotechnology for landscaping in
the Subtropical zone”.
Sterile plants obtained in the process of research are used in laboratory work with
students of the Landscape Construction and Environmental Engineering Faculty of the Sochi
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