Biomedical Engineering Reference
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theoretical plates, and the coefficients of peaks asymmetry did not exceed 1,005. The
sulphuric acid solution (С
H2SO4
=10
-9
mol/l) was used as a mobile phase. The rate of the eluent
feed was 0,5 ml/min, the pressure at the column entrance was 24 atm. The chromatographic
analysis was carried out at a temperature of 30,5°С. The chromatograms were processed by
the method of corrected normalization of the peaks squares.
Monosaccharide composition of common valerian glycans is presented in Table 1.
Table 1. Polysaccharides composition of common valerian
Sugars content (% mass)
ramnose
arabinose
xylose
mannose
galactose
21,2
13,2
5,2
16,2
14,8
Glucose and D-galacturonic acid were detected.
The polysaccharides obtained were tabletted with KBr to analyse their structure by the
method of infra-red spectroscopy. To produce a tablet 1 mm thick and 3mm in diameter 18,4
mg potassium bromide was used. The powder was mixed with substance under study in an
agate mortar. The test quantity of the substance studied was chosen within the range of 0,2-1
% of the immersion substance.
Figure 1. Infra-red spectrum of common valerian polysaccharides.
In the spectrum obtained (Figure 1) there are peaks corresponding to the functioning
groups oscillations characteristic of acid polysaccharides: СООН
(s)
(1540-1580 sm
-1
) (peak
1), СООН
(as)
(1520-1525 sm
-1
) (peak 2), (1040-1050 см
-1
) (peak3),
(1390-1410 см
-1
) (peak 4), С-С (1150 sm
1
(peak 6)). A wide peak corresponding to 1078 sm
-1
(peak 5) allows attributing the polysaccharides obtained to arabinogalactans [8]. The results
of the study [9] of the influence of
Valeriana officinalis L.
carbohydrates on the intestine
C - O - C
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