Biomedical Engineering Reference
In-Depth Information
Pre-adsorption of
positively charged
template protein
Self-assmbly and subsequent surface
graft copolymerization in highly dilute
monomer solution of MAA, AAm,
DMAEMA, and MBA
Template
removal
Rebinding
Figure 12.13
Schematic illustration of the procedure for the imprinting of Lysozyme over
vinyl and carboxyl group-modii ed silica nanoparticles via surface grat copolymerization
in highly dilute solution of functional and crosslinking monomers (Reproduced with
permission from [32]).
by examining the ef ect of several synthesis conditions. Interestingly, the
feed crosslinking degree was found to have a great impact on the thick-
ness of the formed imprinting polymer layers and the recognition prop-
erties of the resulting imprinted materials (Figure 12.13). h e imprinted
particles with a crosslinking degree up to 50% showed the best imprint-
ing ef ect. h e imprinting factor achieved 2.89 and the specii c binding
reached 23.3 mg/g, which are greatly increased compared to those of the
lowly crosslinked imprinted materials reported previously. Moreover, the
relatively high crosslinking degree led to no signii cant retarding of the
binding kinetics to the imprinted particles, and the saturated adsorption
was reached within 10 min.
12.2.1.4 Core-Shell Nanoparticle
Core-shell nanocrystals and/or nanoparticles (CSNPs) are a class of mate-
rials which have properties intermediate between those of small, individual
molecules and those of bulk, crystalline semiconductors. h ey are unique
because of their easily modular properties, which are a result of their size.
h ese nanocrystals are composed of a quantum dot core and a shell of
a distinct semiconducting material (Figure 12.14). h e core and the shell
are typically composed of type II-VI, IV-VI, and III-V semiconductors,
with coni gurations such as CdS/ZnS, CdSe/ZnS, CdSe/CdS, and InAs/
CdSe (typical notation is: core/shell). Organically passivated quantum dots
have low l uorescence quantum yield due to surface-related trap states. h e
