Biomedical Engineering Reference
In-Depth Information
Figure 12.8
Schematic illustration of nanogold-promoted magnetic molecularly
imprinting polymer nanospheres for competitive-type electrochemical detection of
streptomycin (STR) residues by coupling with bioelectrocatalytic reaction of glucose
oxidase (GOX) for signal amplii cation (Reproduced with permission from [27]).
a dynamic range of 0.05-20ng mL
-1
with a detection limit of 10pg mL
-1
STR (at 3s
B
).
Chen
et al.
reported the novel application of m-MIP in the separation
of tetracycline antibiotics from egg and tissue samples [28]. h eextraction
and clean-up procedures were carried out in a single step by blending and
stirring the sample, extraction solvent and polymers. h e analytes can be
transferred from the sample matrix to the polymers directly or through
the extraction solvent as medium. When the extraction was complete,
the polymers adsorbing the analytes were easily separated from the sam-
ple matrix by an adscititious magnet (Figure 12.9). h e analytes eluted
from the polymers were determined by liquid chromatography-tandem
mass spectrometry. h e recoveries ranging from 72.8% to 96.5% were
obtained with relative standard deviations in the range of 2.9-12.3%. h e
limit of detection was less than 0.2 ng g
−1
. h e feasibility of this method
was validated by analysis of incurred egg and tissue samples, and the
results were compared with those obtained by the classical method in
which solvent extraction, centrifugation, and subsequent clean-up and
concentration by solid-phase extraction were applied. h e proposed
method reduced the complicacy of classical method and improved the
reliability of method.
