Biomedical Engineering Reference
In-Depth Information
were also used for the fabrication of m-MIP. Another BHb-imprinted
polystyrene (PS) nanoparticle with magnetic susceptibility have been
synthesized through multistage core-shell polymerization system using
3-aminophenylboronic acid (APBA) as functional and crosslinking
monomers by Lin
et al.
[24]. Super paramagnetic molecularly imprinted
polystyrene nanospheres with poly (APBA) thin i lms have been synthe-
sized and used for the i rst time for protein molecular imprinting in an
aqueous solution. h e magnetic susceptibility is imparted through the
successful encapsulation of Fe
3
O
4
nanoparticles. h e imprinted super
paramagnetic nanoparticles could easily reach the adsorption equilib-
rium and achieve magnetic separation in an external magnetic i eld,
thus avoiding some problems of the bulk polymer.
Gu
et al.
have reported chlorogenic acid imprinted MNPs via water-
in-oil-in-water multiple emulsions suspension polymerization [25]. h is
kind of m-MIPs had the core/shell structure with the size of about 50 nm.
Magnetic susceptibility was given by the successful encapsulation of Fe
3
O
4
nanoparticles with a high encapsulation ei ciency of 19.3 wt%.Z.
Superparamagnetic nanoparticles are currently of great interest for
biomedical applications in both diagnostics and treatment. Lee
et al.
have
reported albumin, creatinine, lysozyme and urea-imprinted polymer
nanoparticles from poly(ethylene-
co
-ethylene alcohol) via phase inver-
sion, with both target molecules and hydrophobic magnetic nanoparticles
mixed within the polymer solution [26]. h e composite m-MIP was used
for separation and sensing of template molecules (e.g., human serum albu-
min) in real samples (urine).
Redox-active m-MIP nanospheres were i rst synthesized and function-
alized with streptomycin templates for highly ei cient electrochemical
determination of streptomycin residues (STR) in food by coupling with
bioelectrocatalytic reaction of enzymes for signal amplii cation by Liu
et al.
[27]. h e m-MIP nanospheres were synthesized by using Au(III)-
promoted molecularly imprinted polymerization with STR templates on
magnetic beads (Figure 12.8).
Based on extraction of template molecules from them MIP surface, the
imprints toward STR templates were formed. h e assay was implemented
with a competitive-type assay format. Upon addition of streptomycin, the
analyte competed with glucose oxidase-labeled streptomycin (GOX-STR)
for molecular imprints on the MIP nanospheres. With the increasing
streptomycin in the sample, the conjugation amount of GOX-STR on the
MIP nanospheres decreased, leading to the change in the bioelectrocata-
lytic current relative to glucose system. Under optimal conditions, the cat-
alytic current was proportional to STR level in the sample, and exhibited
