Biomedical Engineering Reference
In-Depth Information
strategy [18]. In this protocol, the estrone-silica monomer complex was syn-
thesized by the reaction 3-(triethoxysilyl) propyl isocyanate with estrone,
where the template was linked to the silica coating on the iron oxide core
via a thermally reversible bond. h e removal of the template by a simple
thermal reaction produced specii c estrone recognition sites on the sur-
face of silica shell. h e resulting estrone-imprinted polymer coated Fe
3
O
4
magnetic hybrid nanoparticles exhibit a much higher specii c recognition
and saturation magnetization. h e hybrid nanoparticles have been used for
biochemical separation of estrone (Figure 12.6).
In another prospect for MNPs, Kecili
et al.
fabricated superparamag-
netic nanotraps. h e nanoparticle comprises a superparamagnetic iron
oxide nanoparticle core conjugated with trimethoxylsilyl propylmethacry-
late and methacryloylamido serine, methacryloylamido histidine, methac-
ryloylamido glutamic acid monomers, and p-nitrophenyl palmitate which
is a substrate of lipase as a template molecule, which enables the creation of
a lipase active region [19]. h e resulting hybrid superparamagnetic nano-
traps are magnetically separable, highly active, and stable under harsh
conditions. In this study, the advantages of high selectivity of molecular
imprinting technique have been used to obtain mimic lipase for the syn-
thesis of methyl jasmonate and methyl oleate (Figure 12.7).
(
a
)
TEOS
EstSi
Fe
3
O
4
Fe
3
O
4
Fe
3
O
4
DMSO
H2O,
Fe
3
O
4
1
2
3
4
(
b
)
Dibutyltin dilaurate, THF
3-(triethoxysilyl)propyl
isocyante
Estrone
EstSi
Figure 12.6
(a) Multistep synthesis of estrone-imprinted MNPs. Fe
3
O
4
MNPs (1) was
prepared by co-precipitation method and the MNPs surface was then transformed to
silica shell by a sol-gel process using tetraethyl orthosilicate to give Fe
3
O
4
@SiO
2
MNPs
(2). h e Fe
3
O
4
@SiO
2
MNPs reacted with a template-silica monomer complex to produce
silica surface functionalized with estrone-imprinted polymer (3). At er removing the
template estrone by simple thermal reaction, estrone-imprinted polymer coated MNPs
(4) were obtained. (b) Synthesis of template-silica monomer complex (Reproduced with
permission from [18]).
