Biomedical Engineering Reference
In-Depth Information
the electropolymerizable NPs. h e imprinted matrices are implemented
for the sensing of explosives, herbicides, saccharides, and ions. π-Donor-
acceptor interactions, ionic interactions and H-bonds, or ligand-substrate
interactions are used to generate the imprinted sites. h e coupling between
the localized plasmon of the NPs and the surface plasmon wave of the sup-
port is used to amplify the dielectric changes occurring in the NPs matrices
upon the binding of the analytes to the imprinted sites, thus enabling the
surface plasmon resonance (SPR) transduction of the sensing events. h e
imprinted Au NPs matrices demonstrate highly selective, stereoselective,
and chiroselective sensing performance.
In this way to modify the AuNPs, some authors also reported the appli-
cation of gold nanoparticle in electropolymerization with template. Huang
et al.
reported a novel amperometric sensor for bisphenol A (BPA), using
MIP and gold nanoparticle [15]. h is layer is prepared by electropolymer-
ization of 2-aminothiophenol on gold nanoparticle modii ed glassy carbon
electrode in the presence of BPA as template. Cyclic voltammetry (CV)
is use to monitor the process. h e properties of the layer were studied in
the presence of Fe(CN)
6
3-
/Fe(CN)
6
4-
redox couples. h e template and the
non-binding molecules were removed by washing with H
2
SO
4
(0.65 mol
L
-1
) solution. h e linear response range of the sensor was between 8.0×10
-
6
-6.0×10
-2
mol L
-1
, with a detection limit of 1.38×10
-7
mol L
-1
(S/N = 3).
Similarly, some other workers are also trying to apply silver nanoparticles
as MIP platform. Raghu
et al.
have reported a novel electrochemical biosen-
sor for the determination of pyrogallol (PG) and hydroquinone (HQ) based
on the poly l-arginine (poly(l-Arg))/carbon paste electrode (CPE) immobi-
lized with horseradish peroxidase (HRP) and silver nanoparticles (AgNPs)
through the sol-gel entrapment [16]. h e electrochemical properties of the
biosensor were characterized by employing the electrochemical techniques.
h e proposed biosensor showed a high sensitivity and fast response toward
the determination of PG and HQ around 0.18 V. Under the optimized con-
ditions, the anodic peak current of PG and HQ was linear with the concen-
tration range of 8 μM to 30×10
−5
M and 1-150 μM. h e limit of detection
(LOD) and limit of quantii cation (LOQ) were found to be 6.2 μM, 20 μM
for PG and 0.57 μM, 1.92 μM for HQ respectively. h e electrochemical
impedance spectroscopy (EIS) studies have coni rmed that the occurrence
of electron transfer at HRP-SiSG/AgNPs/poly(l-Arg)/CPE was faster.
12.2.1.2
Imprinted Magnetic Nanoparticle
Magnetic nanoparticles (MNPs) are a class of engineered materials of
<100 nm that can be manipulated under the inl uence of an external
