Testicular Xenografting - Male Germline Stem Cells Developmental and Regenerative Potential - page 211

Table 10.2 Outcome of testicular grafting using primate testicular tissue
Primate species and
type of grafting

Age of donors and status
of spermatogenesis

Cryopreservation
before grafting

Treatment
of recipient

Status of grafted testicular tissue and
spermatogenic activity in grafted tissue References

Rathi et al.
( 2008 )

Rhesus monkey
(xenologous)

Infantile/juvenile
(3/6 months)

No Hormone

injections

- Testicular grafts increased in size and showed

normal tubular organization 6 months
post-grafting

- Differentiation of seminiferous tubules and

initiation of spermatogenic activity relied on
hormone treatment

- PMSG and hCG treatment had similar effects

- Grafts from untreated mice contained

spermatogonia as most advanced germ cells

- Following hormone stimulation, tissue from

6-month-old donors showed mature sperm
as most advanced germ cells; 3-months-old
donor tissue developed up to spermatocytes

Human (xenologous) Juvenile (prepubertal,

10-11 years)

No None - Normal somatic tubular morphology Goossens et al.

( 2008 ) - No active spermatogenesis

- Few A-spermatogonia

- No difference between 4 and 9 months

post-grafting

Human (xenologous) Immature/pubertal Ye s None - 6 months post-grafting, 55% of seminiferous

tubules showed normal morphology

Wyns et al.
( 2008 )

- Spermatogonial numbers were reduced to 4%

of fresh donor tissue

- High rate of spermatogonial proliferation

(35%) in grafts

- Spermatocytes and spermatids were detected

as most advanced germ cells in grafted tissue

- Spermatids showed disturbed marker

expression

(continued)