Testicular Xenografting - Male Germline Stem Cells Developmental and Regenerative Potential - page 212

Table 10.2 (continued)

Primate species and
type of grafting

Age of donors and status
of spermatogenesis

Cryopreservation
before grafting

Treatment
of recipient

Status of grafted testicular tissue and
spermatogenic activity in grafted tissue

References

Rhesus monkey
(xenologous)

Pubertal/adult (3/6/11/12
years)

No None - Mostly atrophic tissue with some SCO

tubules in grafts from donors older than 6
years

Arregui et al.
( 2008b )

- Few tubules (0.3%) with spermatocytes in

grafts from 6-year-old donor

- Some tubules with spermatids (0.1/1%) and

spermatocytes (20/60%) at 12 and 24 weeks
post-grafting in grafts from 3-year-old donor

Marmoset (autologous) Immature (4 weeks) and

adult (21 months)

Ye s No - Good recovery and survival of grafts from

immature testes, poor survival and atrophy of
adult testis grafts

Luetjens et al.
( 2008 )

- Most advanced germ cells were

spermatocytes in ectopic grafting sites and
mature spermatids in scrotal grafting sites

- Cryopreservation induced complete atrophy

of testis grafts

Rhesus monkey
(xenologous)

Juvenile (16/19 months) No None, but

exposure of
testicular
tissue
fragments
to
irradiation

- 67-78% of testicular grafts were recovered

after 4 months showing good structural
preservation

Jahnukainen
et al.
( 2007b )

- B-spermatogonia were encountered as most

advanced germ cell types

- Spermatogonial numbers in grafts were

reduced after exposure to 0.5 Gy of
irradiation

- Spermatogonia were almost depleted after

exposure to 1 and 4 Gy of irradiation

- Graft growth was diminished after exposure

to 4 Gy of irradiation