Biomedical Engineering Reference
In-Depth Information
TABLE 4.2
Examples of Biocompatibility Assessment Methods for Delivery Systems that are Responsive to External Stimuli
Stimulus
Source
Biocompatibility
Assessment Method(s)
Time
Point(s)
Biomaterial(s)
Cell Line(s)
Additional Comments
Reference
LAMS-azobenzene
nanoimpeller
Light (413 nm)
Propidium iodide and
Hoechst staining
SW480 and
PANC-1
72 h
Biocompatibility of NPs was assessed at 10 and 100 μg/mL
using latex beads as a reference
Lu et al
.
(2008)
BhcP and ONBP
Light (740 nm)
MTT assay
RAW 264.7
24 h
Biocompatibility of NPs before and after irradiation was
assessed over the concentration range of 1.17-300 μg/mL
Fomina et al
.
(2011)
Light (365 and
435 nm)
None
None
None
Biocompatibility was claimed based on the use of GRAS
reagents
Chen et al
.
(2011)
αα-Cyclodextran/
azobenzene
PEG-anthracene
crosslinker
Light (365 nm)
MTT assay
HCE
3 and 7
days
Biocompatibility assessed by incubating a photogel insert
with cells
Wells et al
.
(2011)
Alkoxylphenacyl-based
polycarbonates
Light (280 nm)
MTT assay, nitrite
production,
in vivo
studies
RAW 264.7
1-3 days,
14 days
Assessed
in vivo
by S.C. and I.V. administration to Balb/c
mice
Wehrung
et al
.
(2013)
Coumarin polyesters
Light (254 and
350 nm)
MTT assay, ROS production,
nitrite production
RAW 264.7
24 h
Biocompatibility assessed before and after irradiation over
the concentration range 5-50 μg/mL
Maddipatla
et al
.
(2013)
O
-Nitrobenzyl alcohol and
bromo-coumarin
Light (350 and
740 nm)
MTT assay
RAW 264.7
24 h
Biocompatibility of NPs before and after irradiation was
assessed over the concentration range of 0.13-100 μg/mL
and compared to PLGA
Lux et al
.
(2012)
Chit-AgNTs
Light (800 nm)
Cell morphology, propidium
iodide and Hoechst staining
HEK and
NCI-H460
24 h
Biocompatibility of nanotriangles was assessed over the
concentration range of 0.3-1.8 μg/mL
Boca et al.
(2011)
Azobenzene-DNA capped
mesoporous silica
Light (365 and
450 nm)
MTT assay
CCRF-
CEM
2 h
Biocompatibility of NPs was assessed at 100 μg/mL
Yuan et al.
(2012)
CPEG-
g
-DNQ
Light (365 nm)
MTT assay
HepG2 and
HUVEC
24 h
Biocompatibility of micelles was assessed over the
concentration range of 10-200 μg/mL
Chen et al.
(2011)
Fluorene and boron-
dipyromethene polymer
Light
(400-800 nm)
MTT assay
A498
24 h
Biocompatibility of NPs was assessed over the
concentration range of 5-20 μM
Chong et al
.
(2012)
4-Hydroxyazobenzene and
pyridine-based azophenols
and azopyrimidine
Light (355 nm)
Colony count
BL21(DE3)
24 h
Biocompatibility of compounds was assessed over the
concentration range of 0.1-30 mM
Garcia-
Amoros
et al
.
(2012)
No material, NIR light
Light (800 nm)
Trypan blue exclusion assay
HeLa
N/A
Biocompatibility of NIR light was assessed over the
energy range of 48-108 μJ/pulse for 1 min, and at 79 μJ/
pulse for 1-7 min
Chen et al
.
(2006)
No material, UV light
Light
(315-400 nm)
Flow cytometry and comet
assay
HaCaT
N/A
Biocompatibility of UV light was assessed over the dose
range of 25-300 kJ/m
2
McMillan
et al
.
(2008)
Fe
2
O
4
/P(NIPPAAm-
co
-
AAc)
Magnetic
Cell morphology, MTS assay
and live-dead staining
L929
48 h
Biocompatibility of nanohydrogels assessed using extracts
incubated with cells at a concentration of 5 mg/mL
Chou et al
.
(2013)
