An Overview of Omics for Wheat Grain Quality Improvement - Crop Improvement: New Approaches and Modern Techniques - page 323

Table 10.2 (continued)

Trait Locus Primer Allele Size (bp) PCR conditions Reference

UMN26F:
CGCAAGACAATATGAGCAAACT

Dy10 397 94 °C/30 s-60 °C/30 s-72 °C/2 m Liu et al. 2008b

UMN26R: TTGCCTTTGTCCTGTGTGC Dy12 415

LMW-GS Glu-A3 F: AAACAGAATTATTAAAGCCGG Glu-A3a 529 94 °C/35 s-60 °C/45 s-72 °C/90 s Wang et al. 2010

R: GGTTGTTGTTGTTGCAGCA
F: TTCAGATGCAGCCAAACAA

Glu-A3b 894 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: GCTGTGCTTGGATGATACTCTA
F: AAACAGAATTATTAAAGCCGG Glu-A3ac 573 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: GTGGCTGTTGTGAAAACGA
F: TTCAGATGCAGCCAAACAA Glu-A3d 967 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: TGGGGTTGGGAGACACATA
F: AAACAGAATTATTAAAGCCGG

Glu-A3e 158 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: GGCACAGACGAGGAAGGTT
F: AAACAGAATTATTAAAGCCGG Glu-A3f 552 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: GCTGCTGCTGCTGTGTAAA
F: AAACAGAATTATTAAAGCCGG

Glu-A3g 1345 94 °C/35 s-60 °C/45 s-72 °C/90 s

R: AAACAACGGTGATCCAACTAA

Glu-B3 F: CACAAGCATCAAAACCAAGA Glu-B3a* 1095 94 °C/35 s-56 °C/35 s-72 °C/90 s Wang et al. 2009b

R: TGGCACACTAGTGGTGGTC
F: ATCAGGTGTAAAAGTGATAG Glu-B3b 1570 94 °C/35 s-56 °C/35 s-72 °C/90 s

R: TGCTACATCGACATATCCA
F: CAAATGTTGCAGCAGAGA Glu-B3c 472 94 °C/35 s-56 °C/35 s-72 °C/90 s

R: CATATCCATCGACTAAACAAA
F: CACCATGAAGACCTTCCTCA Glu-B3d 662 94 °C/35 s-58 °C/35 s-72 °C/90 s

R: GTTGTTGCAGTAGAACTGGA
F: GACCTTCCTCATCTTCGCA Glu-B3e 669 94 °C/35 s-58 °C/35 s-72 °C/90 s

R: GCAAGACTTTGTGGCATT
F: TATAGCTAGTGCAACCTACCAT

Glu-B3fg* 812 94 °C/35 s-63 °C/35 s-72 °C/90 s