An Overview of Omics for Wheat Grain Quality Improvement - Crop Improvement: New Approaches and Modern Techniques - page 322

Table 10.2 An overview of functional (allele specific) markers for grain quality traits in wheat

Trait Locus Primer Allele Size (bp) PCR conditions Reference

HMW-GS Glu-A1 F: CGAGACAATATGAGCAGCAAG Ax2* 344 94 °C/60 s-60 °C/60 s-72 °C/2 m Liu et al. 2008

R: CTGCCATGGAGAAGTTGGA Ax1, AxNull 362

F: ATGACTAAGCGGTTGGTTCTT Ax2* 1319 94 °C/30 s-58 °C/30 s-72 °C/2 m Ma et al. 2003

R: ACCTTGCTCCCCTTGTCTTT

Glu-B1 F: CCTCAGCATGCAAACATGCAGC Bx7 OE 563 95 °C/30 s-58 °C/30 s-72 °C/1 m Butow et al. 2004

R: CTGAAACCTTTGGCCAGTCATGTC
F:CACTGAGATGGCTAAGCGCC Bx6 321 95 °C/30 s-50 °C/30 s-72 °C/1m Schwarz et al. 2004

R: GCCTTGGACGGCACCACAGG
F: ACGTGTCCAAGCTTTGGTTC

Bx7 OE 447 94 °C/35 s-63 °C/30 s-72 °C/60 s Ragupathy et al.

2008

R: GATTGGTGGGTGGATACAGG
F: CCACTTCCAAGGTGGGACTA

Bx7 OE 844 94 °C/35 s-63 °C/30 s-72 °C/60 s

R: TGCCAACACAAAAGAAGCTG
F: CGCAACAGCCAGGACAATT

Bx17 669 94 °C/30 s-58 °C/30 s-72 °C/2m Ma et al. 2003

R: AGAGTTCTATCACTGCCTGGT
F: TTAGCGCTAAGTGCCGTCT By8 527 94 °C/30 s-64 °C/30 s-72 °C/90 s Lei et al. 2006

R: TTGTCCTATTTGCTGCCCTT
F: TTCTCTGCATCAGTCAGGA By9 662 94 °C/30 s-59 °C/30 s-72 °C/90 s

R: AGAGAAGCTGTGTAATGCC nonBy9 707

F: GCAGTACCCAGCTTCTCAA By16, ByNull 94 °C/30 s-62 °C/30 s-72 °C/90 s

R: CCTTGTCTTGTTTGTTGCC Bx20

Glu-D1 UMN25F:

GGGACAATACGAGCAGCAAA

Dx2 299 94 °C/30 s-60 °C/30 s-72 °C/2 m Liu et al. 2008b

UMN25R: CTTGTTCCGGTTGTTGCCA Dx5 281

Forward: CGTCCCTATAAAAGCCTAGC Dx5 478 94 °C/30 s-58 °C/30 s-72 °C/2 m Ma et al. 2003

Reverse:
AGTATGAAACCTGCTGCGGAC