Mutagenesis—A Potential Approach for Crop Improvement - Crop Improvement: New Approaches and Modern Techniques - page 177

Fig. 4.7 Schematic diagram of the overall TILLING and ECOTILLING strategy for plants.
(Modified from Simsek and Kacar 2010 )
have been used. After cleavaging, DNA samples are purified from buffer compo-
nents and then each sample is loaded onto a denaturing polyacrylamide gel. Cleaved
heteroduplexes produced two smaller molecular weight products, one labeled with
IRDye 700 and the other with IRDye 800, whose sizes added up to the size of the
full length product (Till et al. 2007b ).