Design of Insulin Variants for Improved Treatment of Diabetes - Peptide and Protein Design for Biopharmaceutical Applications - page 268

OD
220 nm

Ferritin 500 kDa (a)

Insulin hexamer 36 kDa

Insulin monomer 6 kDa

void

(b)

Insulin 1, no phenol
in SEC eluent

(c)

Insulin 1, 4 mM phenol
in SEC eluent

(d)

Insulin 1, 4 mM phenol
+ 50 mM sorbitol

4 8 12 16 mins

Figure 7.13 Size-exclusion chromatographic analysis showing high-molecular-
weight soluble self-assembly of boronate-polyol insulin in presence of phenol (c),
and the carbohydrate-controlled release of insulin from the self-assembly (d).
Reprinted with permission from the American Chemical Society. Coyright 2005