Biomedical Engineering Reference
In-Depth Information
4.5 Artifacts Induced by the Substitution-Infiltration-Embedding
Technique
Fig. 6.34
Bright-field TEM
image of a slice of
Staphylococcus aureus
embedded first in agar and
then in epoxy resin. This
image shows holes in the
resin due to incomplete
dehydration and embedding
(
arrows
)(
J. Boumendil,
CMEABG UCB Lyon 1
)
4.6 Artifacts Induced by Chemical Fixation
Fig. 6.35
Bright-field TEM
image of an adipocyte cell,
showing a loss of lipids
(
white holes
with
asterisks
)
located in the cytoplasm. The
lipids disappeared during
chemical fixation. As
sectioning was possible, the
resin has filled the residual
holes, but does not show any
contrast (
J. Boumendil,
CMEABG UCB Lyon 1
)
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