Brain Ischemic Injury in Rodents: The Protective Effect of EPO - Tissue-Protective Cytokines: Methods and Protocols - page 95

8. Preparation of thionin stain
Solution A: Buffer solution
1,000 mL distilled H 2 O
7 g Sodium Acetate Solution
2 mL Acetic Acid Solution
Solution B
100 mL distilled H 2 O
1 g thionin
Then add 25 mL solution B with 475 mL solution A
Thionin is hard to dissolve. Use a glass stirring rod or
a stir bar. Do not use a metal rod to stir the thionin.
Filter the thionin solution. All the solution prepara-
tions and the staining are performed under the hood.

Acknowledgments

The work was funded by the Centre National de la Recherche
Scientifique (CNRS), the French Ministère de l'Enseignement
Supérieur et de la Recherche, and the University of Caen Lower
Normandy (UCBN). This work was realized as part of the TC2N
“Trans Channel Neuroscience Network” Interreg IV A 2 Mers
Seas Zeeëns program, “Investing in your future” crossborder
cooperation programme 2007-2013 part financed by the European
Union (European Regional Development Fund).
Conflict of interest: The authors declare that they have no conflict
of interest.

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Neuropharmacology 55:257-270