Systems Biotechnology: Combined in Silico and Omics Analyses for the Improvement of Microorganisms for Industrial Applications - Systems Biology: Networks, Models, and Applications - page 222

Table 7.4 E. coli mutant strains and their genotypes

Strain Genotype or gene knockouts
E. coli W3110 Wild type
E. coli W3110A pykA ::Km r
E. coli W3110G ptsG ::Sp r
E. coli W3110GF ptsG ::Sp r , pykF ::Tc r
E. coli W3110GFA ptsG ::Sp r , pykF ::Tc r , pykA ::Km r
E. coli W3110GFAP ptsG ::Sp r , pykF ::Tc r , pykA ::Km r , pflB ::Cm r
E. coli W3110GFAPL ptsG ::Sp r , pykF ::Tc r , pykA ::Km r , pflB ::Cm r , ldhA ::Pm r
E. coli W3110GFO ptsG ::Sp r , pykF ::Tc r , mqo ::Cm r
E. coli W3110GFH ptsG ::Sp r , pykF ::Tc r , sdh ::Km r
E. coli W3110GFHO ptsG ::Sp r , pykF ::Tc r , sdh ::Km r , mqo ::Cm r
E. coli W3110GFHOE ptsG ::Sp r , pykF ::Tc r , sdh ::Km r , mqo ::Cm r , aceBA ::Pm r
Km r , kanamycin resistance; Sp r , spectinomycin resistance; Tc r , tetracycline resistance;
Cm r , chloramphenicol resistance; Pm r , phleomycin resistance.

system, and eventually to discover a true knowledge map for the
functions of the living system. Although the metabolic system was
the main focus of this chapter, other processes such as signaling and
regulatory networks can be combined to predict more accurate behavior
of the cell under the particular condition.
Systems biotechnology is a discipline that has only recently been
introduced and presents a variety of technical challenges. The central

Table 7.5 Cultivation products formed by E. coli W3110 mutants under
anaerobic condition

Concentration of cultivation
substrate or products (mM) a

Succinic

Strain OD 600 Glucose b Succinic aicd acid ratio c Fold

W3110 1.79 ± 0.11 5.07 ± 0.45 2.43 ± 0.03 0.017 1.00

W3110A 1.62 ± 0.04 5.05 ± 0.56 1.93 ± 0.02 0.012 0.75

W3110G 1.47 ± 0.01 5.66 ± 1.77 2.16 ± 0.08 0.014 0.86

W3110GF 1.46 ± 0.04 4.28 ± 1.42 2.83 ± 0.07 0.019 1.15

W3110GFO 1.49 ± 0.07 4.68 ± 0.35 2.67 ± 0.33 0.018 1.07

W3110GFH 1.35 ± 0.01 4.70 ± 0.39 2.51 ± 0.02 0.017 1.02

W3110GFA 0.73 ± 0.06 20.57 ± 3.02 8.16 ± 0.01 0.137 8.29

W3110GFOH 1.28 ± 0.11 4.82 ± 0.48 2.58 ± 0.03 0.016 1.00

W3110GFOHE 1.27 ± 0.05 4.25 ± 0.27 2.49 ± 0.18 0.017 1.03

W3110GFAP 0.682 ± 0.16 12.2 ± 0.04 8.45 ± 0.05 0.214 12.60

W3110GFAPL 0.05 ± 0.02 41.6 ± 0.8 0.39 ± 0.01 0.19 11.53

a 24 h anaerobic vial cultivation.
b Residual glucose concentration measured (50 mM initial glucose).
c Calculated as succinic aicd/(succinic acid

+ lactate + formate + acetate + ethanol).