Biology Reference
In-Depth Information
complexes, which are often
which allows for an internal
May miss complexes that are
isolated from cell culture
consistency check.
not present under the
using peptide tags that target
Detects real complexes in
experimental conditions.
specific protein sequences,
physiological settings.
Loosely associated components
are purified with 2D gel
Has a low false positive
may be lost during purification.
electrophoresis or liquid
rate.
chromatography. Individual
protein constituents
are then identified by
molecular weight.
Mutagenesis assay
Indicates gene function through
Applicable to any organism
Is redundant, i.e., multiple hits
[25,39]
the exploration of phenotypic
for which transformation and
can occur in a single gene, due
effects of gene mutations.
chromosomal integration
to the intrinsic randomness
Short exogenous gene
of DNA can occur.
of insertion events.
sequences are randomly
Inserted DNA can be tailored
Is incapable of generating a
inserted into a genome, and
to meet experimental goals,
complete collection of gene
can mutate a functional gene.
e.g., determining expression
functionality, though a
The mutation itself serves as
and protein localization.
spectrum of alleles that can
a molecular marker for the
Does not depend on
display different phenotypic
subsequent identification of
predetermined and possibly
and/or localization characteristics
the disrupted gene. A variant
erroneous assignments of
is often generated.
of this method involves
gene. position since it can
creating a site-specific
be a random approach.
insertional mutation.
Easy to determine which gene
is mutated by virtue of the
insertion sequence, which
serves as a molecular marker
in the disrupted gene.
Continued
Search WWH ::
Custom Search