Mapping the Genotype–Phenotype Relationship in Cellular Signaling Networks: Building Bridges Over the Unknown - Systems Biology: Networks, Models, and Applications - page 144

Table 5.2 ( Continued )

Name Description Advantages Disadvantages References

extracted from the cell is The probe is affected by the

hybridized on it. The method of (1) RNA isolation and

resulting array identifies (2) incorporation of the available

genes that are up- and down- labeling agents.

regulated under the given
experimental conditions.

Fluorescent resonance Identifies proteins that interact Capable of yielding real-time May not accurately identify [36,37]

energy transfer (FRET) with each other. Proteins are data, including precise interaction if the hybrid

tagged with fluorescent location and time of proteins fail to attain

markers that generate visible interaction. appropriate orientation and

fluorescent resonant energy Effectively detects separation distance.

transfer (FRET) when they intramolecular activity, Subject to noise from unpaired

come into close proximity i.e., interactions wherein the proteins that are colocalized.

with one another. donor and acceptor

fluorescent protein are fused
to the same host protein.

Gene knockout Indicates the phenotypic Effective tool for Difficult to characterize [38,39]

function of a specific gene. investigating global effects functions of lethal knockouts.

The gene of interest is of individual genes.

replaced with a null mutation,
and the resulting cellular
behavior is observed to
identify functions of the
deleted gene.