Toxicology and Risk Assessment - Nanotechnology and the Environment - page 217

Rats SWCNT 30.nm 1.or.5.mg/kg.

intratracheally.
instilled;.evaluated.24.
hr,.1.week,.1.month,.
and.3.months.post.
exposure

Cytotoxicity/lung Transient.inlammatory.and.cell.

injury.effects,.non-dose-
dependent.series.of.multi-
focal.granulomas.

Physiological.relevance.of.
these.indings.should.be.
determined.by.conducting.an.
inhalation.toxicity.study.

[25]

Mouse.L929.
ibrosarcoma
Rat.C6.glioma
U251.human.glioma

C60.fullerence
C60(OH) n .—.
polyhydroxylated.
fullerene

100.nm
<5.nm

1.or.1000.(g/mL Oxidative.

stress/cytotoxicity

Cytotoxic.action.reached.
maximum.after.6.hr.with.C60;.
minimal.cytotoxicity.for.the.
same.time.period.for.
C60(OH) n ;.Reactive.oxygen.
species.(ROS).not.produced.in.
cells.treated.with.C60(OH) n ;.
rapid.increase.of.ROS.in.cells.
treated.with.C60.

Results.demonstrate.that.C60.
is.at.least.three.orders.of.
magnitude.more.toxic.than.
C60(OH) n .

[49]

Rats C60.fullerenes 160.±.50.nm 0.2,.0.4,.1.5,.or.3.0.

mg/kg.intratracheally.
instilled;.lung.tissue.
evaluated.1.day,.1.
week,.1.month,.and.3.
months.post.
instillation

Oxidant.and.
glutathione.
endpoints;.
bronchoalveolar.
lavage.(BAL).
luid.biomarkers;.
lung.tissue

Transient.inlammatory.and.cell.
injury.at.1.day.post.exposure,.
not.different.from.controls.at.
other.post.exposure.times;.
BAL.biomarkers.increased.in.
1.5.and.3.0.mg/kg.dose.
groups.at.1.day.and.3.months.
post.exposure;.no.adverse.
lung.tissue.effects.at.3.months.
post.exposure.at.any.dose.

Results.not.consistent.with.
results.reported.for.in.vitro.
studies;.such.indings.
highlight.the.dificulty.in.
extrapolating. in vitro .effects.
to. in vivo .effects.

[50]