Probiotic Applications in Cold Water Fish Species - Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics - page 237

Dry feed (10 8 CFUg -1 ) given for
3 weeks; fish challenged with V.
anguillarum (10 7 CFUml -1 ,1h)

(7 wks) C. divergens (from cod or

salmon intestine)

LAB colonization in intestines
and/or pyloric caeca after 3
weeks; reduced cumulative
mortality 12 days post-infection
using the salmon strain, but not
after 24 days

Gildberg and Mikkelsen
(1998)

Dry feed (10 7-9 CFUg -1 ), single
or mixed probiotics

(28or55days) Arthrobacter and Enterococcus

spp. (ALL)

Faster growth of Enterococcus fed
juveniles and lower Vibrio gut
counts than control; both
probiotics modified gut
microbiota, enhanced juvenile
growth and survival

Lauzon et al. (2010d)

Dry feed (10 6 CFUg -1 ) for 40
days

(20or40days) Psychrobacter GP12 or

Pseudomonas GP21 spp.
(AUT)

Increased amylolytic and
cellulolytic activities in cod
intestine only after 40 days for
both strains; enhanced chitinase
and protease activity in GP12
fed fish

Lazado et al. (2012)

In vitro test
(24 h)

Psychrobacter sp., Shewanella
sp., Photobacterium sp. or
Vibrio sp. (AUT)

Incubation of HK cells with
heat-inactivated gut-derived
bacteria (10 6 CFUml -1 )

Differential expression of immune
response genes related to
bacterial defence and
inflammation; the
Photobacterium strain did not
elicit much response

Caipang et al. (2010)

10 7 cells (live or heat inactivated)
added to the primary cultures of
HK leukocytes

In vitro test
(24 h)

Psychrobacter GP 12 and
Pseudomonas GP21 spp.
(AUT)

Cod intestinal bacteria regulated
some genes responsible for
immune and antioxidant
defences in cod HK leukocytes;
bacterial viability influenced the
extent of genes expression
obtained

Lazado et al. (2010)

(continued overleaf )