Methodological Approaches Used to Assess Fish Gastrointestinal Communities - Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics - page 104

Table 5.1 (continued)

Fish species Methodology Notes Cultivability Reference

∗ ∗

Carp
Cyprinus carpio

55-100% Sugita et al . (1981)

Cultivable levels of 1.0 × 10 6 to
2.0 × 10 9 CFUg -1 and total counts of
3.3 × 10 9 to 4.7 × 10 9 cells g -1

Bambooleaf wrasse
Pseudolabrus
japonicas

Cultivable bacteria: 1/20PYBG
agar, 14 days, 25 ∘ C, aerobic
Total bacteria: DAPI

0.022-48.6% Sugita et al . (2005)

Cultivable levels of log 6.86 CFUg -1
and total counts of log 10.74 cells g -1
(note: n = 1)

Cultivable bacteria: PYBG agar
medium
Total bacteria: DAPI

ca. 0.1% Shiina et al . (2006)

Cultivable levels of 3.6 × 10 6 to
3.4 × 10 9 CFUg -1 and total counts of
3.2 × 10 9 to 4.2 × 10 9 cells g -1

Cupid wrasse
Thalassoma cupido

Cultivable bacteria: 1/20PYBG
agar, 14 days, 25 ∘ C, aerobic
Total bacteria: DAPI

0.095-80.9% Sugita et al . (2005)

Cultivable bacteria: PYBG agar
medium
Total bacteria: DAPI

Cultivable levels of log 5.73-6.81
CFUg -1 and total counts of log
10.82-10.93 cells g -1 (note: n = 2)

ca. 0.00001-
0.0001%

Shiina et al . (2006)

Cultivable levels of 7.7 × 10 5 to
9.3 × 10 7 CFUg -1 and total counts of
2.9 × 10 9 to 4.3 × 10 9 cells g -1

Scribbled toby
Canthigaster rivulata

Cultivable bacteria: 1/20PYBG
agar, 14 days, 25 ∘ C, aerobic
Total bacteria: DAPI

0.018-2.7% Sugita et al . (2005)

Cultivable levels of 2.3 × 10 7 to
2.5 × 10 9 CFUg -1 and total counts of
3.4 × 10 9 to 1.0 × 10 10 cells g -1

Grass puffer
Takifugu niphobles

Cultivable bacteria: 1/20PYBG
agar, 14 days, 25 ∘ C, aerobic
Total bacteria: DAPI

0.3-50.4% Sugita et al . (2005)

Cultivable bacteria: PYBG agar
medium
Total bacteria: DAPI

Cultivable levels of log 6.13-8.68
CFUg -1 and total counts of log
10.67-11.18 cells g -1

ca. 0.1-1% Shiina et al . (2006)

Cultivable levels of 6.2 × 10 4 to
4.6 × 10 7 CFUg -1 and total counts of
9.4 × 10 9 to 2.8 × 10 10 cells g -1

Largescale blackfish
Girella punctata

Cultivable bacteria: 1/20PYBG
agar, 14 days, 25 ∘ C, aerobic
Total bacteria: DAPI

0.0009-0.22% Sugita et al . (2005)

Cultivable bacteria: PYBG agar
medium
Total bacteria: DAPI

Cultivable levels of log 4.00-6.45
CFUg -1 and total counts of log
10.81-11.27 cells g -1

ca. 0.000001-0.1% Shiina et al . (2006)