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In-Depth Information
samples, Fig.
33.1a
). Transgenic fragments were found only in milk from goats fed
GM soybean. In the case of the 35S promoter, the frequency was 38-75% in fresh
samples and 25% in stored samples; CP4 EPSPS were amplified in 38-50% of fresh
samples and 25% of stored samples (Fig.
33.1b
).
33.4 Discussion
According to other authors (Einspanier et al.
2001
; Phipps et al.
2003
; Poms et al.
2003
; Tudisco et al.
2010
), it has been confirmed that the detection of specific gene
fragments is more difficult than that of nonspecific fragments. In addition, our
results confirm the possibility to detect transgenic fragments in milk samples from
animals fed GM feed, as previously reported (Tudisco et al.
2010
). Concerning food
traceability, the influence of sample storage on the concentration as well as on the
quality of DNA extracted has been demonstrated; indeed, both parameters were
decreased in the pellet of somatic cells stored at
20
C.
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