Biology Reference
In-Depth Information
To evaluate the effi cacy of the fusion phage to inhibit the virus propagation
in
ovo
, different pfu (10
8
-10
13
/100 μl) of recombinant fusion phages were mixed with
constant amount of virus (16 HAU) and injected into the allantoic cavity of embryo-
nated chicken eggs. After 3 days, the allantoic fl uid was harvested and the HA titer was
measured. The fusion phage FP-P1 reduced the viral titer in the allantoic fl uid upto
four fold at the concentration more than 10
13
pfu/100 μl (Figure 5). Based on the dose
response curve, the IC
50
for FP-P1 was approximately 5 × 10
11
pfu/100 μl.
Figure 5.
Antiviral activity of fusion phages
in ovo
. The fusion phage concentration needed to inhibit
50% of the virus growth was determined using different concentrations of recombinant phages FP-
P1. Experiments were done in triplicates and the error bars represent the standard error of the mean.
*, statistical significance (P < 0.05) (The SEM value is not shown for some data as there was no
variation between repeated experiments).
Besides, to determine whether these peptides inhibit the virus replication specifi -
cally, these peptides (linear, cyclic and FP-P1) were tested for inhibitory effects against
NDV strain AF2240. None of these molecules do not posses signifi cant (ANOVA, p =
0.596) inhibitory effect against NDV replication (Figure 6).
Figure 6.
Effect of peptides against NDV. Cyclic, linear and FP-P1 at 100 μM concentrations were
analyzed for their inhibitory ability against NDV in embryonated chicken eggs. Viral titers in the
allantoic fluid were measured as HA units. Results are shown as the mean of three independent
experiments and error bars represent the standard deviation of the mean. None of the peptides
showed a statistically significant result (ANOVA, p = 0.596).
