Novel Anti-viral Peptide Against Avian Influenza Virus H9N2 - Recent Advances in Microbiology - page 42

Figure 3. Antiviral activity of fusion phages in vitro . The MDCK cells were inoculated with untreated
virus (AIV H9N2) or virus treated with increasing concentration of fusion phages and the cell viability
was determined by MTT assay. Results shown are the mean of three trials +/- SD.
Antiviral Activity of Peptides and Fusion Phages in Ovo
Peptides were evaluated for their antiviral activity in ovo against AIV H9N2. Briefly,
different concentrations of both cyclic and linear peptides (0.00, 0.001, 0.01, 0.1, and
1 mM) were mixed with constant amount of virus (8 HAU) and injected into allantoic
cavity of embryonated chicken eggs. After 3 days, the allantoic fluid was harvested
and the HA titer was determined. Complete inhibition was observed at the concentra-
tion 1 mM (Figure 4). The IC50 values of both cyclic and linear peptides were 48 μM
and 71 μM respectively.
Figure 4. Antiviral activity of peptides in ovo . The peptide concentration needed to inhibit 50% of
the virus growth was determined using different concentrations of peptides. Experiments were done
in triplicates and the error bars represent the standard error of the mean. *, statistical significance (P
< 0.05) (The SEM value is not shown for other values as there was little variation between repeated
experiments).