Biology Reference
In-Depth Information
at m/z 731.5 (diagnostic of PA) and 886 (PGS) that were reported for
Halorubrum
sp. [19] could not be detected in any of our samples. Negative ion ESI-MS (Figure
2A, B, and C) identified major signals corresponding to PG m/z 805.7, PGP-Me m/z
899.5 (as monocharged peak), 449 (as bicharged peak) and S-DGD m/z 1055.7 (as
monocharged peak). The diagnostic peak of archaeal cardiolipin (BPG) at m/z 760 (as
bicharged peak) and 1,521 (monocharged peak) was detected only as a small signal in
the three TPL extracts.
ARC Characterization
As revealed by transmission electron microscopy, the two ARC preparations were
multilamellar, with a mean size of 564 ± 22 nm and zeta-potential near to -50 mV. The
BSA incorporation did not modify size or zeta-potential, the protein/lipid ratio was 20
μg/mg and the encapsulation efficiency around 3-4%.
ARC Uptake by Cells and Cellular Toxicity
None of the ARC or HSPC:cholesterol liposomes significantly reduced the viability of
non phagocytic cells (Vero cell line) upon 24 hr incubation (Figure 3A). On the other
hand, 10 μg/ml ARC-
GC
was non cytotoxic but the higher concentrations reduced
cell viability by 25%, while increased concentrations (up to 100 μg/ml) of ARC-
BM
and HSPC:cholesterol liposomes did not affect cultured macrophages (J-774 cell line)
(Figure 3B).
Figure 3.
Viability of Vero (A) and J-774 cells (B) upon 24 hr incubation with ARC-
GC
, ARC-
BM
or
HSPC:cholesterol liposomes, as function of concentration. Values represent the average of triplicates
± S.D. ANOVA, *
p
< 0.05.
