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(Stewart). Two calibration curves were prepared employing dry mass of TPL as stan-
dards that resulted linear between 10-40 μg for Bötcher and 20-400 μg for Stewart,
with correlation coeffi cients exceeding 0.997, for each source of TPL.
On the other hand, calibration curves using inorganic phosphate (NaH
2
PO
4
) as
standard were prepared with the aim of determining the phosphate percentage of ar-
chaeal lipids by the Bötcher method. A linear plot of dry mass of TPL versus μg inor-
ganic phosphate was determined and the relative amount of phosphate in the extracted
TPL was similar for both isolates, in the order of 6.25% w/w.
ESI-MS Polar Lipids Profile
The Electrospray ionization-mass spectrometry (ESI-MS) spectrum (negative ions)
analyses of the TPL of
H. tebenquichense
,
BM
, and
GC
showed three main peaks at
m/z 805, 899, and 1055.9 (Figure 2). In addition,
GC
displayed intense ion peak at
m/z 1,521 that was less intense for
H. tebenquichense
and
BM
extracts. The peaks
Figure 2.
Electrospray ionization-mass spectrometry (ESI-MS) analysis of polar lipids from (A)
H.
tebenquichense
, (B)
BM
, and (C)
GC
extract.