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In-Depth Information
s
(1000, 2000, 10,000, and 14,000) was also performed [28]. When
PTHFs with
Preparation of inclusion complexes using PTHFs with various
M
n
s = 1000 and 2000 were used as the guest polymers,
inclusion complexes were obtained, which were confirmed by
means of the XRD and
M
n
1
H NMR measurements. In contrast, PTHFs
with higher
s, such as 10,000 and 14,000, were not dispersed
well in the buffer of the polymerization solvent, and accordingly,
inclusion complexes were not formed from these PTHFs. To obtain
the inclusion complexes using these PTHFs, the polymerization
was performed in the following two-phase system. The PTHF was
dissolved in diethyl ether and the buffer was added to the solution
(1:5, v/v). Enzymatic polymerization of Glc-1-P then occurred with
vigorous stirring to disperse the diethyl ether phase in the buffer.
The XRD patterns of the products from the PTHFs with
M
n
s equal to
10,000 and 14,000 obtained by the two-phase system indicated the
formation of the inclusion complexes.
The effect of the end groups of the telechelic PTHFs as the guest
polymers was investigated [28]. The end groups employed were
the hydroxy (-OH, as aforementioned), methoxy (-OCH
M
n
), ethoxy
3
(-OCH
Ph) groups. The content of PTHF
in the product obtained from the methoxy-terminated PTHF was
close to that obtained from the hydroxy-terminated PTHF, indicating
the formation of the inclusion complex. However, the content of
PTHF in the product obtained from the ethoxy-terminated PTHF
was much lower than that obtained from the aforementioned guest
PTHFs. In addition, when the benzyloxy-terminated PTHF was used,
no inclusion complex was obtained. These results indicated that the
formation of the inclusion complexes was strongly affected by the
bulkiness of the end groups of the PTHFs.
CH
), and benzyloxy (-OCH
2
3
2
7.3
Preparation of Inclusion Complex Using
Other Polyethers as Guest Polymers
To investigate the effect of the alkyl chain lengths of the guest
polyethers for the formation of inclusion complexes, the enzymatic
polymerization was carried out in the presence of polyethers with
different alkyl chain lengths, i.e., PTHF (Fig. 7.2a), poly(oxetane)
(POXT, Fig. 7.2b), and poly(ethylene glycol) (PEG) [28]. The
structures of the products were characterized by means of the XRD
