Biology Reference
In-Depth Information
the molecular weight of PTHF equal to 4000 was used. The molecular
weight of the amylose was 12200-14600, corresponding to 9.9-11.9
nm of molecular length in helical form. However, the molecular
weight of the included PTHF was ca. 2800, suggesting that the chain
length is ca. 23.0 nm. Therefore, one PTHF molecule is probably
included by two amylose molecules on the average.
Mixing amylose and PTHF in a buffer did not form the inclusion
complex. This observation suggested that the inclusion complex
formed during enzymatic polymerization. To study the relation
between the formation of the inclusion complex and the enzymatic
polymerization process, the following experiment was performed
(Fig. 7.7). When PTHF was added to the reaction solution immediately
after the general enzymatic polymerization of Glc-1-P had started, an
identical inclusion complex to that mentioned earlier was obtained.
However, the contents of PTHF in the products decreased as the time
between the start of the enzymatic polymerization and the addition of
the PTHF to the solution was increased. These observations revealed
that the inclusion complexes were not formed after the production
of amyloses with relatively higher molecular weights. These results
indicated that polymerization proceeded with the formation of the
inclusion complex.
Figure 7.7
Investigation of relation between the formation of the
inclusion complex and phosphorylase-catalyzed enzymatic
polymerization process.
