Biomedical Engineering Reference
In-Depth Information
Fig. 9.17.
Amplitudes reconstructed from single recorded holograms in different
focus planes (
left
: unfocussed reconstruction in the hologram plane;
right
: numer-
ically focused). (
a
),(
b
) Semitransparent USAF 1951 test chart (
×
40 microscope
optic, NA = 0.6) and (
c
),(
d
) Living human pancreas tumor cell Patu8988S (
×
100
oil immersion optic, NA = 1.3) [28]
9.5.4 Digital Holographic Phase Contrast Microscopy
of Living Cells
Investigations on living pancreas tumor cells [39, 40] were carried out to
demonstrate the potential of digital holographic microscopy for the visual-
ization of toxin-induced morphology changes. Therefore, pancreatic tumor
cells were exposed at 37
◦
C to taxol. Digital holograms of selected cells were
recorded continuously over 4 h. Figure 9.18 shows the results. In the upper
row of Fig. 9.18, the obtained unwrapped phase distributions at
t
=0,
t
= 78,
