Taurine and Chinese Traditional Medicine Accelerate Alcohol Metabolism in Mice - Advances in Experimental Medicine and Biology - page 20

Table 3.1 Experimental design

Group Alcohol intake Treatment (i.g.)

I 30 min before Water

II 30 min before 2% taurine

III 30 min before 2% T+CTM

IV 30 min after Water

V 30 min after 2% taurine

VI 30 min after 2% T+CTM

3.2 Methods

3.2.1 Antialcohol Intoxication Effects of Taurine and Chinese

Traditional Medicine on Mice

60 Kunming mice were divided into six groups randomly. Mice in each group were
intragastrically (i.g.) administered with alcohol (i.g., 60%, 0.4 ml) 30 min before or
after i.g. with water (C), 2% taurine (T), or 2% taurine coadministration with
Chinese traditional medicine (T+CTM), the volume of which were all 0.4 ml.
The disappearance of the body-righting reflex which was used to determine the
intoxication of mice was recorded when the mice were kept in a supine position
without turned over for more than 30 s. Durations between alcohol intake and
intoxication (tolerance time), intoxication and recovery (maintenance time) were
recorded. The experimental design is shown in Table 3.1 .

3.2.2 Determination of Alcohol Concentration

in Blood After Alcohol Intake

60 Kunming mice were divided into three groups and were treated as group I, II, and
III as shown in Table 3.1 . Blood were collected from each group 20, 30, 90 and
150 min after alcohol intake. Alcohol concentrations were detected by way of gas
chromatography.

3.2.3 Determination of Hepatic Activities of ADH and ALDH

Liver were collected at the same time of blood collection. Hepatic activities of
alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) were
detected by chromatometry using spectrophotometer.